Anti-Acinus(CP)Antibody
CatalogNo.:2215
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnewinducerofchromatincondensationwasrecentlyidentifiedanddesignatedAcinus(forApoptoticChromatinCondensationInducerintheNucleus).
Acinusiscleavedbycaspase-3andanadditionalproteasetogenerateasmallactivepeptide,p17,whichcauseschromatincondensationinvitrowhenitisaddedtopurfiednuclei.Acinusalsoinducesapoptoticchromatincondensationincells.
Acinusisubiquitouslyexpressed.ThreedifferentsplicedformsofAcinushavebeenidentifiedinhumanandmouseandaredesignatedAcinusL,AcinusS,andAcinusS’.
Antigen:Peptidecorrespondingtoaa
1065-1080ofhumanAcinusL,aa307-322ofhumanAcinusSandaa338-353ofhumanAcinusS’whichareidenticaltothoseofmouseAcinus.ThesesequencesarelocatedneartheC-terminusofthecleavedactivepeptidep17.
HostSpecies:Rabbit
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanandmouseAcinus(220kD).
APPLICATIONSImmunoblotting:useat1-10ug/ml
WesternblotanalysisofAcinusinK562wholecelllysatewithAcinusantibody(CP)at1μg/ml.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofK562cellsusingAcinusantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:K562cellsorwholecelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
