Anti-Acinus(CT)Antibody
CatalogNo.:2217
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnewinducerofchromatincondensationwasrecentlyidentifiedanddesignatedAcinus(forApoptoticChromatinCondensationInducerintheNucleus).
Acinusiscleavedbycaspase-3andanadditionalproteasetogenerateasmallactivepeptide,p17,whichcauseschromatincondensationinvitrowhenitisaddedtopurfiednuclei.Acinusalsoinducesapoptoticchromatincondensationincells.
Acinusisubiquitouslyexpressed.ThreedifferentsplicedformsofAcinushavebeenidentifiedinhumanandmouseandaredesignatedAcinusL,AcinusS,andAcinusS’.
Antigen:Peptidecorrespondingtoaa
1329-1341ofhumanAcinusL,aa571-583ofhumanAcinusSandaa602-614ofhumanAcinusS’whichareidenticaltothoseofmouseAcinus.
HostSpecies:Rabbit
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanandmouseAcinus(220kD).
APPLICATIONSImmunoblotting:useat0.5-1ug/ml
WesternblotanalysisofAcinusinK562wholecelllysatewithAcinusantibody(CT)at0.5μg/ml.
Immunocytochemistry:useat0.5-1ug/ml.
ImmunocytochemicalstainingofK562cellsusingAcinusantibodyat0.5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:K562cellsorwholecelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
