CatalogNo.:2241

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

AnewinducerofchromatincondensationwasrecentlyidentifiedanddesignatedAcinus(forApoptoticChromatinCondensationInducerintheNucleus).

Acinusiscleavedbycaspase-3andanadditionalproteasetogenerateasmallactivepeptide,p17,whichcauseschromatincondensationinvitrowhenitisaddedtopurfiednuclei.Acinusalsoinducesapoptoticchromatincondensationincells.

Acinusisubiquitouslyexpressed.ThreedifferentsplicedformsofAcinushavebeenidentifiedinhumanandmouseandaredesignatedAcinusL,AcinusS,andAcinusS’.

Antigen:Peptidecorrespondingtoaa994-1009ofhumanAcinusL,aa236-251ofhumanAcinusSandaa267-282ofhumanAcinusS’whichareidenticaltothoseofmouseAcinus.ThesesequencesarelocatedneartheN-terminusofactivepeptidep17.

HostSpecies:Rabbit

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizeshumanandmouseAcinus(220kD).

Immunoblotting:useat1-10ug/ml

WesternblotanalysisofAcinusinK562wholecelllysatewithAcinusantibody(NP)at1μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:K562wholecelllysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.