ORDERINGINFORMATION

CatalogNo.:2051

Format:100uginPBS,pH7.4,0.02%sodiumazide.Purifiedbypeptideimmunoaffinitychromatography.

BACKGROUND

TNFα,Notchanditsliganddeltaareallmembrane-boundmoleculeswhicharecleavedbyproteasestoreleasematureproteinsorfunctionalreceptors.ADAM10,ametalloprotease-disintegrininthefamilyofmammalianADAM(foradisintegrinandmetalloprotease)wasrecentlyfoundtocleaveTNFα,Notchanditsliganddelta.Thegenesencodinghuman,mouse,andbovineADAM10havebeenclonedanddesignatedADAM10,Kuzbanian(KUZ),andMADM,respectively.Thesegenesareexpressedinavarietyofhumanandbovinetissues.

SPECIFICATIONSUMMARY

Antigen:Peptidecorrespondingtoaa732-748ofhumanADAM10.Thissequenceisidenticaltothoseofbovineandratanddiffersfrommousebyoneaminoacid.

AccessionNo.:NP_001101

HostSpecies:Rabbit

Specificity:Human.Inimmunoblotsabandof85kDaisdetectedandmayrepresentprecursor.Lowermwbandsaredetectedinsomecelllines,includingJurkat,andmayrepresenttheprocessedmatureprotein.

APPLICATIONS

Immunoblotting:useat1-10ug/ml.

Immunofluorescence:useat10ug/ml.

Immunocytochemistry:useat2ug/ml.

WesternblotanalysisofADAM10inJukatwholecelllysate.

ImmunofluorescentdetectionofADAM10inK562cells.

ImmunochemicaldetectionofADAM10inK562cells.

Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodycanbestoredat4oCforthreemonthsandforoneyearat-20oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.