Anti-AIF(CT)Antibody
CatalogNo.:2301
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelproteinthatcauseschromatincondensationandDNAfragmentationhasbeendesignatedApoptosisInducingFactor(AIF).AIFlocalizesinmitochondriaandtranslocatestothenucleuswhenapoptosisisinduced.Thiseventisfollowedbythereleaseofchytochromecandcaspase-9frommitochondria.AIFishighlyconservedbetweenhumanandmouseandwidelyexpressedindifferentcelltypesandtissues.
Antigen:Peptidecorrespondingtoaa593-606attheC-terminusofhumanAIF.ThissequenceisidenticaltothoseofmouseandratAIF.
HostSpecies:Rabbit
Preservatives:0.02%sodiumazide
Thisantibodyrecognizeshuman,mouse,andratAIF(67kD).
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
APPLICATIONSImmunoblotting:useat0.5-2ug/ml.
WesternblotanalysisofAIFinK562celllysatewithAIFantibodyat(A)0.5,(B)1,and(C)2μg/ml.
Immunocytochemistry:useat5ug/ml.
ImmunocytochemicalstainingofAIFinK562cellswithAIFantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:K562cellsorwholecelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
