Anti-AIF(IN)Antibody
CatalogNo.:2267
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
AnovelproteinthatcauseschromatincondensationandDNAfragmentationhasbeendesignatedApoptosisInducingFactor(AIF).AIFlocalizesinmitochondriaandtranslocatestothenucleuswhenapoptosisisinduced.Thiseventisfollowedbythereleaseofchytochromecandcaspase-9frommitochondria.AIFishighlyconservedbetweenhumanandmouseandwidelyexpressedindifferentcelltypesandtissues.
Antigen:Peptidecorrespondingtoaa517-531ofhumanAIF.ThissequenceisidenticaltothoseofmouseandratAIF.HostSpecies:Rabbit
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratAIF(67kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1-10ug/ml
WesternblotanalysisofAIFinK562celllysate(A),ratheart(B),andmouseheart(C)tissuelysateswithAIF(IN)antibodyat1μg/ml.
Immunohistochemistry:useat10ug/ml
ImmunohistochemistryofAIFinhumanretinawithAIF(IN)antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:WholecelllysatefromK562cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
