Anti-AIF(NT)Antibody
CatalogNo.:2239
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelproteinthatcauseschromatincondensationandDNAfragmentationhasbeendesignatedApoptosisInducingFactor(AIF).AIFlocalizesinmitochondriaandtranslocatestothenucleuswhenapoptosisisinduced.Thiseventisfollowedbythereleaseofchytochromecandcaspase-9frommitochondria.AIFishighlyconservedbetweenhumanandmouseandwidelyexpressedindifferentcelltypesandtissues.
Antigen:Peptidecorrespondingtoaa109-122attheN-terminusofhumanAIF.
HostSpecies:Rabbit
Preservatives:0.02%sodiumazide
ThisantibodyrecognizeshumanAIF(67kDa).
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
APPLICATIONSImmunoblotting:useat1-10ug/ml.
WesternblotanalysisofAIFinK562celllysatewithAIF(NT)antibodyat1μg/ml.
Immunocytochemistry:useat2-5ug/ml.
ImmunocytochemicalstainingofAIFinJurkatcellswithAIF(NT)antibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:K562ofJurkatcellsorwholecelllysates.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
