Annexin1belongstoafamilyofCa(2+)-dependentphospholipidbindingproteinswhichhavemolecularweightsofapproximately35-40kDaandarelocalizedonthecytosolicfaceofthe

plasmamembrane.Annexin1,anendogenousanti-inflammatoryprotein,hasrolesinmanydiversecellularfunctions,suchasmembraneaggregation,inflammation,phagocytosis,proliferation,andapoptosis.Annexin1alsopossessesphosphorylationsitesforimportant

proliferativesignalingmoleculese.g.,EGFreceptortyrosinekinaseandPKC.ThissuggeststhatAnnexin1mayhavearoleincertainsignalingpathwaysimportantincancer.However,theexactmechanismsthroughwhichAnnexin1exertssomeorallofitseffectsarestillnotclearlyunderstood,andmayrevealimportantfunctionsofAnnexin1intumOrigenesisandcancerdevelopment.

ThisantibodyrecognizeshumanandbovineAnnexin1.

Immunoblotting:useat1-10ug/ml.Abandof~39kDaisdetected.

Immunohistochemistry:useat2-20ug/ml.

Immunoprecipitation:useat1-2ugper100-500ugoftotalprotein.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Reconstituteindistilledwater.

Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.