Anti-Apaf1(CT)Antibody
CatalogNo.:2015
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ThemammalianhomologuesofthekeycelldeathgeneCED-4inC.eleganshasbeenidentifiedrecentlyfromhumanandmouseanddesignatedApaf1(forapoptosisprotease-activatingfactor1).Apaf1bindstocytochromec(Apaf2)andcaspase-9(Apaf3),whichleadstocaspase-9activation.Activatedcaspase-9inturncleavesandactivatescaspase-3,oneofthekeyproteasesresponsIBLefortheproteolyticcleavageofmanykeyproteinsinapoptosis.
Apaf1canalsoassociatewithcaspase-4andcaspase-8.Apaf1isubiquitouslyexpressedinhumantissues.
Antigen:Peptidecorrespondingtoaa1158-1177ofhumanApaf1.Thissequence
differsfromthatofmouseApaf1byoneaminoacid.
HostSpecies:Rabbit
Preservatives:0.02%sodiumazide
Thisantibodyrecognizeshuman,mouse,andratApaf1(130kDa).
APPLICATIONSImmunoblotting:useat1-10ug/ml
WesternblotanalysisofApaf1inhumanhearttissuelysatewithApaf1(CT)antibodyat1μg/mlintheabsence(A)orpresence(B)ofblockingpeptide.
Immunohistochemistry:useat10-20ug/ml
ImmunohistochemistryofApaf1inhumanskintissuewithApaf1(CT)antibodyat20μg/ml.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Humanhearttissuelysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
