CatalogNo.:2412

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

ThemammalianhomologuesofthekeycelldeathgeneCED-4inC.eleganshasbeenidentifiedrecentlyfromhumanandmouseanddesignatedApaf1(forapoptosisprotease-activatingfactor1).Apaf1bindstocytochromec(Apaf2)andcaspase-9(Apaf3),whichleadstocaspase-9activation.Activatedcaspase-9inturncleavesandactivatescaspase-3,oneofthekeyproteasesresponsIBLefortheproteolyticcleavageofmanykeyproteinsinapoptosis.

Apaf1canalsoassociatewithcaspase-4andcaspase-8.Apaf1isubiquitouslyexpressedinhumantissues.

Antigen:Peptidecorrespondingtoaa12-28ofhumanApaf1.Thissequenceisidenticalinhumanandmouse.

HostSpecies:Rabbit

Preservatives:0.02%sodiumazide

Thisantibodyrecognizesfull-lengthmouseandhumanApaf1(115-130kDa).

Immunoblotting:useat1-2ug/ml

WesternblotanalysisofApaf1inK562celllysatewithApaf1(NT)antibodyat1μg/mlinthe(A)absenceand(B)presence

ofblockingpeptide.

Immunohistochemistry:useat1-2ug/ml

ImmunohistochemistryofApaf1inhumanhearttissuewithApaf1(NT)antibodyat1μg/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysateofHeLaorK562cells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.