CatalogNo.:2413

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

AnovelCARDdomain-containingproteinwasrecentlyidentifiedanddesignatedARC(apoptosisrepressorwithCARD).ARCinteractswithcaspase-2and-8andinhibitsenzymaticactivityofcaspase-8.ARCsuppressesapoptosisinducedbycelldeathadaptersFADDandTRADDandbycelldeathreceptorsFas,TNFR-1,andDR3.

ThemessengerRNAofARCisprimarilyexpressedinskeletalmuscleandcardiactissue.

Antigen:Peptidecorrespondingtoaa191-208ofhumanARC.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizesfull-lengthARC(25kD).

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat1-10ug/ml.

WesternblotanalysisofARCinHeLa(H),KB(K),andA549(A)wholecelllysateswithARCantibodyat1ug/ml.

Immunohistochemistry:useat5ug/ml.

A

B

ImmunohistochemistryofARCinhumanhearttissue

(A)andskeletalmuscle(B)withARCantibodyat5μg/ml.

Positivecontrol:WholecelllysateofHeLa,KB,andA549cells.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.