Anti-ARC(NT)Antibody
CatalogNo.:2185
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelCARDdomain-containingproteinwasrecentlyidentifiedanddesignatedARC(apoptosisrepressorwithCARD).ARCinteractswithcaspase-2and-8andinhibitsenzymaticactivityofcaspase-8.ARCsuppressesapoptosisinducedbycelldeathadaptersFADDandTRADDandbycelldeathreceptorsFas,TNFR-1,andDR3.ThemRNAofARCisprimarilyexpressedinskeletalmuscleandcardiactissue.
Antigen:Peptidecorrespondingtoaa2-18ofhumanARC.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratARC(25kD).
APPLICATIONSImmunoblotting:useat2ug/ml
WesternblotanalysisofARCinHeLacelllysatewithARCantibodyat2ug/mldilution.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:WholecelllysateofHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
