CatalogNo.:2287

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

AnovelCARD-containingproteinwasrecentlyidentifiedanddesignatedASC(orTMS1).ASCinducesapoptosisbyactivatingcaspase-9andinhibitssurvivalofhumanbreastcancercells.ASCiswidelyexpressedinhumanandmousetissues.

Antigen:Peptidecorrespondingtoaa182-195ofhumanASC.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizeshumanASC(25kDa).

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofASCinHL60wholecelllysateintheabsence(A)orpresence(B)ofblockingpeptidewithASCantibodyat1μg/ml.

Immunocytochemistry:useat5ug/ml.

ImmunocytochemistryofASCinHL60cellswithASCantibodyat5μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysateofHL60cells.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.