Anti-ASK1Antibody
CatalogNo.:1151
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelMAPkinasekinasekinase(MAPKKK)wasrecentlyidentifiedanddesignatedASK1(apoptosissignal-regulatingkinase1)andMAPKKK5.ASK1activatestwodifferentsubgroupsofMAPKK,MKK4andMKK6,whichinturnactivatec-junN-terminalkinase(JNK)andp36MAPkinase.ASK1/MAPKKK5isactivatedbyTNFRandFasthroughtheinteractionwithmembersoftheTRAFfamilyandFas-associatedproteinDaxx.
OverexpressionofASK1inducesapoptoticcelldeath.AcatalyticallyinactiveformofASK1inhibitsTNF-inducedapoptosis.ASK1isexpressedinavarietyofhumanandmousetissues.
Antigen:Peptidecorrespondingtoaa1356-1375ofhumanASK1.Thispeptidesequenceisdifferentfromthatofmouseinthelasttwoaminoacids.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanASK1(155kDa).
APPLICATIONSImmunoblotting:useat0.5-1ug/ml
WesternblotanalysisofASK1inSW1353wholecelllysatewithASK1antibodyat0.5g/ml.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofASK1inA431cellswithASK1antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.
Positivecontrol:WholecelllysatefromSW1353cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
