Anti-BAFF(CT)Antibody
CatalogNo.:2221
Size:100ugIgGin200ulPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelmemberoftheTNFsuperfamilywasrecentlyidentifiedanddesignatedBAFF(BCellActivatingFactoroftheTNFFamily)orBlyS(BLymphocyteStimulator),TALL-1(TNF-andApoL-RelatedLeukocyte-ExpressedLigand),andTHANK(TNFHomologuethatActivatesApoptosis,NF-Bandc-junN-terminalKinase).BAFFisdesignatedaBcellstimulatorsinceitinducesBcellproliferationandimmunoglobulinsecretion.BAFFanditsreceptorsareinvolvedinthedevelopmentofsystemiclupuserythematosusandotherBcell-associatedautoimmunediseases.
Antigen:Peptidecorrespondingtoaa254-269attheC-terminusofhumanBAFF.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratBAFF(approx.38kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofBAFFinhumanHL60celllysate(H)andmurinespleentissuelysate(M)withBAFFantibodyat1μg/ml.
Immunocytochemistry:useat1ug/ml.
ImmunocytochemicalstainingofBAFFinHL60cellswithBAFFantibodyat1μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.
Positivecontrol:WholecelllysatefromHL60cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
