Anti-Bcl10(NT)Antibody
CatalogNo.:2161
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelCARDcontainingproteinwasrecentlyidentifiedanddesignatedBcl10,CIPER,mE10,CARMEN,andCLAP.Bcl10isacellularhomologoftheequineherpesvirus-2E-10gene.OverexpressionofBcl10inducesJNK,p38,andNF-Bactivation.Bcl10interactswithcaspase-9,enhancespro-caspase-9processing,andinducesapoptosisthroughcaspase-9activation.Bcl10exhibitsavarietyofmutationsinMALTlymphomaandinBandTcelllymphomasindicatingthatitmaybecommonlyinvolvedinthepathogenesisofhumantumors.Bcl10isexpressedinmanyhumanandmurinetissuesandcelllines.
Antigen:Peptidecorrespondingtoaa5-19ofhumanBcl10.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanBcl10(31kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofBcl-10inRajiwholecelllysateintheabsence(A)orpresence(B)ofblockingpeptidewithBcl10antibodyat1g/ml.
Immunocytochemistry:useat1ug/ml.
ImmunocytochemicalstainingofBcl10inRajicellswithBcl10antibodyat1μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.
Positivecontrol:WholecelllysateofRajicells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
