CatalogNo.:2414

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

BACKGROUND

AnovelBH3domain-containingproteinwasrecentlyidentifiedanddesignatedBimorBODfromhuman,mouse,andrat.Bim/BODinteractswithdiversemembersinthepro-survivalBcl-2subfamilyincludingBcl-2,Bcl-xL,andBcl-w.Bim/BODinducesapoptosis.ThemessengerRNAofBimisubiquitouslyexpressedinmultipletissuesandcelllines.

Antigen:Peptidecorrespondingtoaa22-40ofhumanBim.Thesequenceisidenticaltothatofmouseandisdifferentbyoneaminoacidfromthatofrat.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizesfull-lengthBim(23kDa)ofhuman,mouse,andrat.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofBiminK562(K)andA549(A)wholecelllysateswithBimantibodyat1μg/ml.

Immunocytochemistry:usea10-20ug/ml

ImmunohistochemicalstainingofBiminhumanskincancercellswithBimantibodyat20μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.

Positivecontrol:WholecelllysateofhumanK562cells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.