Anti-Bmf(CT)Antibody
CatalogNo.:11001
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:Peptidecorrespondingtoaa171-184ofhumanBmf(accessionno.
NP_277038).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThisantibodyrecognizesanepitopeattheC-terminusofhumanBmf(Bcl-2-ModifyingFactor),anovelBH3-onlyprotein.Inhealthycells,Bmfassociateswiththedyneinlightchain2componentofthemyosinVmotorsandissequesteredbythecell’sactinCytoskeleton.Disruptionoftheactincytoskeleton,eitherbydepolymerizationofactinfilamentsorbydetachmentofcellsfromtheextracellularmatrix,triggersreleaseandactivationofBmf,initiaingthedownstreamapoptoticprogram.Bmfisconstitutivelyexpressedinmanytissues.
APPLICATIONSImmunoblotting:useat2.5-5ug/ml.Abandof25kDaisdetected.
WesternblotanalysisofBmfinHepG2celllysatewithBmfantibodyat(A)2.5and(B)5μg/ml.
Immunofluorescence:useat10ug/ml.
ImmunofluorescentstainingofBmfinhumankidneytissuewithBmfantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.
Positivecontrol:HepG2or293celllysates.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
