Anti-Bmf(NT)Antibody
CatalogNo.:11002
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:Peptidecorrespondingtoaa2-14ofhumanBmf(accessionno.NP_277038).HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThisantibodyrecognizesanepitopeattheN-terminusofhumanBmf(Bcl-2-ModifyingFactor),anovelBH3-onlyprotein.Inhealthycells,Bmfassociateswiththedyneinlightchain2componentofthemyosinVmotorsandissequesteredbythecell’sactinCytoskeleton.Disruptionoftheactincytoskeleton,eitherbydepolymerizationofactinfilamentsorbydetachmentofcellsfromtheextracellularmatrix,triggersreleaseandactivationofBmf,initiaingthedownstreamapoptoticprogram.Bmfisconstitutivelyexpressedinmanytissues.
APPLICATIONSImmunoblotting:useat2ug/ml.Abandof25kDaisdetected.
WesternblotanalysisofBmfexpressioninhumanHepG2(lane1)and293(lane2)celllysateswithBmfantibodyat2μg/ml.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofBmfinHeLacellswithBmfantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.
Positivecontrol:HepG2or293celllysates.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
