Anti-Bnip3L(IN)Antibody
CatalogNo.:2289
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelBH3domain-containingproteinwasrecentlyidentifiedanddesignatedBnip3L,Bnip3,orNix.Bnip3LisahomologoftheE1B19K/Bcl-2bindingandpro-apoptoticproteinBnip3.OverexpressionofBnip3Linducesapoptosis.Bnip3LinteractswithandovercomessuppressionbyBcl-2andBcl-xL.Bnip3Lislocalizedinmitochondria.ThemessengerRNAofBnip3Lisubiquitouslyexpressedinhumantissues.
Bnip3LandBnip3formanewsubfamilyofthepro-apoptotic-mitochondrialproteins.
Antigen:Peptidecorrespondingtoaa77-92ofhumanBnip3L(accessionno.
AF067396)Thisaminoacidsequenceisidenticaltothatofmouse.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizesmonomerichumanandmouseBnip3L.Abandof40kDaisdetectedinimmunoblots.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofBnip3LinK562wholecelllysateintheabsence(A),orpresence(B)ofimmunogenicpeptidewithBnip3Lantibodyat1μg/ml.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofhumankidneytissueusingBnip3Lantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:WholecelllysateofhumanK562cells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
