Anti-CAD(CT)Antibody
CatalogNo.:2011
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.ThesedeathsignalsfinallycausethedegradationofchromosomalDNAbyactivatedDNase.AmouseDNasethatcausesDNAfragmentationwasidentifiedrecentlyanddesignatedCAD(caspaseactivateddeoxyribo-nuclease).ActivationofCAD/DFF40,whichcausesDNAdegradation,isthehallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa314-329ofmouseCAD(accessionno.O54788).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthmouseCAD(40kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofCADinmurinelung(L)andkidney
(K)tissuelysateswithCADantibodyat1μg/ml.
Immunohistochemistry:useat1ug/ml.
ImmunohistochemicalstainingofCADinmousekidneytissuewithCADantibodyat1μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Mouselungorspleentissuelysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
