CatalogNo.:2416

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.ThesedeathsignalsfinallycausethedegradationofchromosomalDNAbyactivatedDNase.AmouseDNasethatcausesDNAfragmentationwasidentifiedrecentlyanddesignatedCAD(caspaseactivateddeoxyribo-nuclease).ActivationofCAD/DFF40,whichcausesDNAdegradation,isthehallmarkofapoptoticcelldeath.

Antigen:Peptidecorrespondingtoaa205-222ofmouseCAD(accessionno.O54788).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizesfull-lengthmouseCAD(40kDa).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat2ug/ml.

WesternblotanalysisofCADinmousekidneytissuelysatewithCADantibodyat2μg/ml.

Immunohistochemistry:useat2ug/ml.

ImmunohistochemicalstainingofCADinmousekidneytissuewithCADantibodyat2μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:Mouselungtissuelysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.