Anti-CARD9Antibody
CatalogNo.:11003
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:Peptidecorrespondingtoaa521-536ofhumanCARD9(accessionno.AF31187).
ThissequencediffersfromthatofratCARD9bytwoaminoacids.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThisantibodyrecognizeshumanCARD9,anovelCARD(CaspaseRecruitmentDomain)-containingproteinthatinteractswiththeCARDactivationdomainofBcl-10.CARD9associateswithBcl-10toformacomplexwithincells.CARD9inducesapoptosisandactivatesNF-B.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat2-3ug/ml.Abandof59kDaisdetected.
WesternblotanalysisofCARD9expressioninhumanMDA-MB-361(A)andPC-3(B)celllysatewithCARD9antibodyat2.5μg/ml.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofCARD9inK562cellswithCARD9antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:PC-3celllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
