Anti-Caspase-12(IN)Antibody
CatalogNo.:2327
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Threedistinctsignalingpathwaysleadtoprogrammedcelldeath.Thedeathreceptorandmitochondrialpathwaysarethemainpathwaysinwhichtheproteasescaspase-8andcaspase-9,respectively,areinvolved.Endoplasmicreticulum(ER)stressisthethirdpathway,andcaspase-12isinvolved.Caspase-12,localizedintheER,isactivatedbyERstressandmediatesERstress-inducedapoptosis.SeveralproteinsthatareassociatedwithAlzheimer’sdisease,including-secretase,presenilin,and-amyloidprecursorprotein(APP),co-localizeintheERwithcaspase-12.Caspase-12isubiquitouslyexpressedinmousetissues.
Antigen:Peptidecorrespondingtoaa100-116ofmousecaspase-12(accessionno.
CAA73532).
StABIlizers:NonePreservatives:0.02%sodiumazide.HostSpecies:Rabbit
Thisantibodyrecognizeshuman,mouse,andratcaspase-12(approx.53kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofCaspase-12inmousebraintissuelysateintheabsence(A)orpresence(B)ofblockingpeptidewithCaspase-12antibodyat1μg/ml.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofCaspase-12inmouseliverusingCaspase-12antibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Tissuelysateofmousebrain.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
