Anti-Caspase-12(NT)Antibody
CatalogNo.:2325
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Threedistinctsignalingpathwaysleadtoprogrammedcelldeath.Thedeathreceptorandmitochondrialpathwaysarethemainpathwaysinwhichtheproteasescaspase-8andcaspase-9,respectively,areinvolved.Endoplasmicreticulum(ER)stressisthethirdpathway,andcaspase-12isinvolved.Caspase-12,localizedintheER,isactivatedbyERstressandmediatesERstress-inducedapoptosis.SeveralproteinsthatareassociatedwithAlzheimer’sdisease,including-secretase,presenilin,and-amyloidprecursorprotein(APP),co-localizeintheERwithcaspase-12.Caspase-12isubiquitouslyexpressedinmousetissues.
Antigen:Peptidecorrespondingtoaa2-17attheN-terminusofmousecaspase-12(accessionno.CAA73532).
HostSpecies:Rabbit
Preservatives:0.02%sodiumazide
Thisantibodyrecognizeshuman,mouse,andratcaspase-12(approx.53kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofCaspase-12inhuman(A)andmouse(B)spleentissuelysateswithCaspase-12antibodyat1μg/ml.
Immunohistochemistry:useat10ug/ml.
ImmunohistochemicalstainingofCaspase-12in
humanhearttissuewithCaspase-12antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Tissuelysateofmousebrain.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
