ORDERINGINFORMATION

Catalognos.:11024-50(50ug)11024-200(200ug)

Format:ProteinA-purifiedantibodyinPBS,50%glycerol,0.09%sodiumazide.

BACKGROUND

Caspasesarecysteineproteasesthatcleaveaftercertainaspartateresiduesandhavebeenidentifiedasmediatorsofapoptosis.Caspasesaresynthesizedasinactivezymogensthatcanbecleavedtoformactiveenzymesfollowingtheinductionofapoptosisbystressordeathreceptors.Initiatorcaspases(e.g.caspase-8and-10)areactivatedbydimerizationofthezymogenonadedicatedadaptorprotein.Theseactivatedinitiatorcaspasesin-turncleavedownstreameffectororexecutionercaspases(e.g.caspase-3,-6,and-7)inacascade-likemanner,whichcleavekeycellularproteinsthatleadtothemorphologicalchangesassociatedwithapoptoticcelldeath.

SPECIFICATIONSUMMARY

Antigen:Recombinantfulllengthhumancaspase-3.

Accessionno.:P42574

GeneID:836

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Specificity:Thisantibodyrecognizescaspase-3inhuman,mouse,rat,bovine,canine,chicken,guineapig,hamster,monkey,pig,rabbit,andyeast.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~36kDaisdetected.

Immunohistochemistry:useat10ug/ml

Detectionofcaspase-3inhumanspleen.

Detectionofcaspase-3inJurkatcelllysate(lane2),staurosporine-treatedJurkatcelllysate(lane3),MCF-7celllysate(negativecontrol,lane4),HeLacelllysate(lane5),ratspleenlysate(lane6),mousespleenlysate(lane7).

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.