CatalogNo.:11025

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Caspasesareafamilyofcysteineproteases.Caspase-4wasinitiallyidentifiedasahomologousproteintoCaspase-1andtheC.elegansCed-3whichcouldinduceapoptosisintransfectedcells.MorerecentstudieshaveshownthatitcanbeactivatedbyERstressandhasbeensuggestedtobeinvolvedinmultipleneuronalpathologiessuchasAlzheimer'sdisease.

Antigen:Syntheticpeptidecorrespondingto16aminoacidsattheamino-terminusofhumanCaspase-4(accessionno.

AAA86890).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizeshumanandmouseCaspase-4(36kDa).

Immunoblotting:useat1ug/ml.

WesternblotanalysisofCaspase-4inRamoscellswithcaspase-4antibodyat(A)0.5and(B)1μg/ml.

Positivecontrol:Ramoscelllysate.

Immunohistochemistry:useat2ug/ml.

ImmunohistochemicalstainingofmousespleenwithCaspase-4antibodyat2μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.