Anti-Caspase-8Antibody
CatalogNo.:11026
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Caspasesareafamilyofcysteineproteases.Caspase-8isaninitiatorcaspasethatwasidentifiedasamemberoftheFas/APO-1death-inducingsignalingcomplex.TheadaptormoleculeFADDcouplesprocaspase-8totheFasreceptordeathdomain;subsequentoligomerization
promotesprocaspase-8autoactivation.FLIP,acatalyticallyinactivecaspase-8-likemoleculeinhibitstheseinteractionsandthuscaninhibitapoptosis.
Antigen:Syntheticpeptidecorrespondingto16aminoacidsattheC-terminusofhumanCaspase-8isoformA(accessionno.NP_001219).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratCaspase-8(18kDa).
APPLICATIONSImmunoblotting:useat0.5-2ug/ml.
WesternblotanalysisofCaspase-8inJurkatcelllysatewithCaspase-8antibodyat(A)0.5,(B)1,and(C)2μg/ml.
Positivecontrol:Jurkatcelllysate.
Immunocytochemistry:useat2ug/ml.
ImmunocytochemicalstainingofCaspase-8inJurkatcellswithCaspase-8antibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
