Anti-Caspase-9(I16)Antibody
CatalogNo.:2417
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.Caspase-9andApaf-1bindtoeachother,whichleadstocaspase-9activation.Caspase-9isalsoactivatedbygranzymeBandCPP32.
Activatedcaspase-9cleavesandactivatescaspase-3,aproteaseresponsIBLeforproteolyticcleavageofmanykeyproteinsinvolvedinapoptosis.Caspase-9playsacentralroleincelldeathinducedbyawidevarietyofapoptosisinducersincludingTNF,TRAIL,anti-CD95,FADD,andTRADD.Caspase-9isexpressedinavarietyofhumantissues.
Antigen:Peptidecorrespondingtoaa41-56ofhumancaspase-9(accessionno.P55211).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhumancaspase-9(46kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofCaspase-9inHeLawholecelllysatewithCaspase-9antibodyat1ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:WholecelllysatefromHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
