productdatasheet
CatalogNo.:11093
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
DNAfragmentation,inducedbyDNAfragmentationfactor(DFF),isoneofthecriticalstepsinapoptosis.DFFiscomposedoftwosubunits,a40-kDacaspase-activatednuclease(DFF40)anda45-kDainhibitor(DFF45).
Recentlyanovelfamilyofcelldeath-inducingDFF45-likeeffectors(CIDEs)hasbeenidentified.HumanCIDE-3isanovelCIDE.Twotranscripts,CIDE-3andCIDE-3alpha,arepresentinHepG2andA375cells.Consistentwithitschromosomelocalizationat3p25,aregionassociatedwithhighfrequencylossofheterozygosityinmanytumors,CIDE-3mayplayanimportantroleinpreventionoftumOrigenesis.
Liang,Letal.(2003)BiochemJ370:195-203.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SPECIFICATIONSUMMARYImmunogen:Syntheticpeptidecorrespondingtoaminoacids10-25(LLYPKSLSRHVSVRTS)ofhuman
CIDE-3conjugatedtoKLHattheC-terminus.
HostSpecies:Rabbit
StABIlizers:NonePreservatives:None.Availableonrequest.
ThisantibodyrecognizeshumanCIDE-
3.SequencehomologytothemouseCIDE-3homolog,FSP27,is93%.
Immunoblotting:useat1:500dilution.Abandofapproximately27kDaisdetected.
Positivecontrol:HepG2celllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
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