Anti-CIDE-A(CT)Antibody
CatalogNo.:2085
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
DFF45-relatedproteinsCIDE-AandCIDE-B(forcelldeath-inducingDFF-likeeffectorAandB)wererecentlyidentified.CIDEcontainsanewtypeofdomaintermedCIDE-NwhichhashighhomologywiththeregulatorydomainsofDFF45/ICADandDFF40/CAD.ExpressionofCIDE-AinducesDNAfragmentationandactivatesapoptosiswhichisinhibitedbyDFF45.CIDE-Aisexpressedinmanytissues
Antigen:Peptidecorrespondingtoaa200-217ofhumanCIDE-A(accessionno.AF041378)HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanCIDE-A(23kDA)anddoesnotcross-reactwithCIDE-B.
APPLICATIONSImmunoblotting:useat0.5ug/ml.
Immunohistochemistry:useat5ug/ml.
WesternblotanalysisofCIDE-Ainhumanbraintissuelysateintheabsence(A)orpresence(B)ofimmunogenicpeptidewithCIDE-Aantibodyat0.5ug/ml.
ImmunohistochemicalstainingofCIDE-AinhumanbraintissuewithCIDE-Aantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Humanbraintissuelysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
