Anti-CIDE-B(CT)Antibody
CatalogNo.:2091
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
DFF45-relatedproteinsCIDE-AandCIDE-Bhaverecentlybeenidentified.CIDEcontainsanewtypeofdomaintermed
CIDE-N,whichhashighhomologywiththeregulatorydomainsofDFF45/ICADandDFF40/CAD.ExpressionofCIDE-Binducesapoptosis,whichisinhibitedbyDFF45.
CIDE-BisaDFF45-inhibitableeffectorthatpromotescelldeathandDNAfragmentation.CIDE-Bisexpressedmainlyinliverandatlowerlevelsinspleen,kidney,peripheralbloodlymphocytes,andbonemarrow.
Antigen:Peptidecorrespondingtoaa204-219ofmouseCIDE-B(accessionno.
AF041377).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizesmouseCIDE-B(25kDa)anddoesnotcross-reactwithCIDE-A.
APPLICATIONSImmunoblotting:useat2ug/ml.
WesternblotanalysisofCIDE-BinmurinelivertissuelysatewithCIDE-Bantibodyat2ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.
Positivecontrol:Tissuelysateofmouseliver.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
