CatalogNo.:2323

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Certainserine/threonineproteinkinases,suchasRIPandDAPkinase,aremediatorsofapoptosis.DAPkinase(DAPK)isapro-apoptotic,calcium-regulatedserine/threoninekinasecontainingadeathdomain.ExpressionofDAPKinducescelldeathandsuppressesoncogenictrans-formation.DAPKmediatesIFN-inducedapoptosis.ADAPK-relatedproteinwasrecentlydescribedanddesignatedDAPK2,orDRP-1.ExpressionofDAPK2inducesapoptosis.ThemessengerRNAforDAPK2isexpressedinmultiplehumantissues.

Antigen:Peptidecorrespondingtoaa356-370ofhumanDAPK2(accessionno.BAA88063).Thissequenceisidenticaltothatofmouse.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizeshuman,mouse,andratDAPK2(approx.42kDa)anddoesnotcross-reactwithDAPK.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticapplications.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofDAPK2inA431(H),mousespleen(M),andratkidney(R)lysateswithDAPK2antibodyat1μg/ml.

Immunohistochemistry:useat2ug/ml.

ImmunohistochemicalstainingofDAPK2inmousespleencellswithDAPK2antibodyat2μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysatefromA431cells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.