Anti-Daxx(CT)Antibody
CatalogNo.:2411
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelFasbindingproteinwasrecentlyclonedfromhumanandmouseanddesignatedDaxx.DaxxbindsspecificallytotheFasdeathdomain,enhancesFasinducedapoptosis,andactivatestheJunN-terminalkinase(JNK)pathway.Daxxiswidelyexpressedinfetalandadulthumanandmousetissues,indicatingitsimportantfunctioninFassignalingpathways.
Antigen:Peptidecorrespondingtoaa722-740attheC-terminusofhumanDaxx(accessionno.CAG33366).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhuman,monkey,andmouseDaxx(120kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1:500-1:1,000dilution.
WesternblotanalysisofDaxxin293wholecelllysatewithDaxxantibodyat1ug/ml.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofDAXXinHeLacellswithDAXXantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:WholecelllysatefromHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
