Anti-DcR1(ED)Antibody
CatalogNo.:2179
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
TRAIL/Apo2LisamemberoftheTNFfamilythatinducesapoptosisinavarietyoftumorcelllines.DR4andDR5aretherecentlyidentifiedfunctionalreceptorsforTRAIL.TwodecoyreceptorsforTRAILhavebeendesignatedDcR1/TRID/TRAIL-R3/LITandDcR2/TRAIL-R4/TRUNDD.DcR1hasanextracellularTRAIL-bindingdomainbutlacksanintracellularsignalingdomain.Itisaglycophospholipid-anchoredcellsurfaceprotein.DcR1transcriptsareexpressedinmanynormalhumantissuesbutnotinmostcancercelllines.OverexpressionofDcR1doesnotinduceapoptosisbutattenuatesTRAIL-inducedapoptosis.
Antigen:Peptidecorrespondingtoaa149-167attheextracellulardomain(ED)ofhumanDcR1precursor(accessionno.
AF012536).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratDcR1(65kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofDcR1inHeLawholecelllysatewithDcR1antibodyat1g/ml.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofDcR1inHeLacellswithDcR1antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:WholecelllysatefromHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
