Anti-DcR1(ED2)Antibody
CatalogNo.:2299
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
TRAIL/Apo2LisanewmemberoftheTNFfamilythatinducesapoptosisinavarietyoftumorcelllines.DR4andDR5aretherecentlyidentifiedfunctionalreceptorsforTRAIL.TwodecoyreceptorsforTRAILhavebeendesignatedDcR1/TRID/TRAIL-R3/LITandDcR2/TRAIL-R4/TRUNDD.
DcR1hasanextracellularTRAIL-bindingdomainbutlacksanintracellularsignalingdomain.Itisaglycophospholipid-anchoredcellsurfaceprotein.DcR1transcriptsareexpessedinmanynormalhumantissuesbutnotinmostcancercelllines.
OverexpressionofDcR1doesnotinduceapoptosisbutattenuatesTRAIL-inducedapoptosis.
Antigen:Peptidecorrespondingtoaa111-123attheextracellulardomain(ED)ofhumanDcR1precursor(accessionno.
AAB67104).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratDcR1(65kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofDcR1inHeLacell(A),mouse
(B)andrat(C)livertissuelysateswithDcR1antibodyat1μg/ml.
Immunofluorescence:useat10ug/ml.
ImmunofluorescentstainingofDcR1inratlivertissuewithDcR1antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:WholecelllysatefromHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures
