CatalogNo.:2419

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

TRAIL/Apo2LisanewmemberoftheTNFfamilyandinducesapoptosisinavarietyoftumorcelllines.DR4andDR5aretherecentlyidentifiedfunctionalreceptorsforTRAIL,andDcR1/TRIDisa"decoyreceptor".AnothermemberoftheTRAILreceptorfamilywasmorerecentlyidentifiedanddesignatedDcR2,TRAIL-R4,orTRUNDD.DcR2hasanextracellularTRAIL-bindingdomainbutlacksanintracellulardeathdomainanddoesnotinduceapoptosis.

LikeDR4andDR5,DcR2transcriptiswidelyexpressedinnormalhumantissues.

OverexpressionofDcR2attenuatesTRAIL-inducedapoptosis.

Antigen:Peptidecorrespondingtoaa249-263ofhumanDcR2precursor(accessionno.

Q9UBN6).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizesfull-lengthDcR2(34kDa).

Immunoblotting:useat1ug/ml.

WesternblotanalysisofDcR2inHeLacelllysatewithDcR2antibodyat1ug/ml.

Immunocytochemistry:useat10ug/ml.

ImmunocytochemicalstainingofHeLacellswithDcR2antibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysatefromHeLacells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.