CatalogNo.:2227

Size:100ugIgGin200ulPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.Anovelproteinthatinteractswiththedeatheffectordomainofcapase-8andcaspase-10hasbeendesignatedDEDAFandisdedenticaltothetranscriptionalrepressorRYBP.DEDAFisexpressedinmultipletissuesandcelllinesandappearstobeinvolvedintheregulationofbothcytoplasmicandnucleareventsofapoptosis.

Antigen:Peptidecorrespondingtoaa215-229ofhumanDEDAF(accessionno.AF179286).

Thissequenceisidenticaltothatofmouse.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizeshuman,mouse,andratDEDAF(32kDa).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofDEDAFexpressioninhumanA549(laneA),HepG2(laneB),andmouse3T3(laneC)celllysateswithDEDAFantibodyat1μg/ml.

Immunohistochemistry:useat10ug/ml.

ImmunohistochemicalstainingofDEDAFinmouselivertissuewithDEDAFantibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysatefromA549orHepG2cells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.