CatalogNo.:2409

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

AhumanDNAfragmentationfactor(DFF)whichiscleavedbycaspase-3duringapoptosiswasidentifiedrecently.ThemousehomologueofhumanDFFwasidentifiedasaDNaseinhibitorandwasdesignatedICAD.UponcleavageofDFF/ICAD,acaspaseactivateddeoxyribonuclease(CAD)isreleasedandactivatedandeventuallycausesthedegradationofDNAinnuclei.CleavageofCADinhibitormoleculeDFF/ICAD,whichcausesDNaseactivationandDNAdegradation,isahallmarkofapoptoticcelldeath.

Antigen:Peptidecorrespondingtoaa313-331attheC-terminusofhumanDFF45(accessionno.NP_004392).

HostSpeciec:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizesnon-cleaved(45kDa)andcleavedDFF45.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofDFF45inHeLa(H),Jurkat(J),A431(A),andK562(K)wholecelllysatewithanti-DFF45(CT)at1ug/ml.

Immunocytochemistry:useat5ug/ml.

ImmunocytochemicalstainingofDFF45inHeLacellswithDFF45antibodyat5μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysatefromHeLacells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.