Anti-DFF(NT)Antibody
CatalogNo.:2410
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AhumanDNAfragmentationfactor(DFF)whichiscleavedbycaspase-3duringapoptosiswasidentifiedrecently.ThemousehomologueofhumanDFFwasidentifiedasaDNaseinhibitorandwasdesignatedICAD.UponcleavageofDFF/ICAD,acaspaseactivateddeoxyribonuclease(CAD)isreleasedandactivatedandeventuallycausesthedegradationofDNAinnuclei.CleavageofCADinhibitormoleculeDFF/ICAD,whichcausesDNaseactivationandDNAdegradation,isahallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa2-21attheN-terminusofhumanDFF45(accessionno.NP_004392).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesnon-cleaved(45kDa)andcleavedDFF.
APPLICATIONSImmunoblotting:useat2ug/ml.
WesternblotanalysisofDFF45inHeLa(H),K562(K)Jurkat(J),andRaji(R)celllysateswithDFF45antibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:WholecelllysatefromHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
