Anti-DFF40/CAD(IN)Antibody
CatalogNo.:2155
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.ThesedeathsignalsfinallycausethedegradationofchromosomalDNAbyactivatedDNase.AmouseDnasethatcausesDNAfragmentationwasidentifiedrecentlyanddesignatedCAD(forcaspaseactivateddeoxy-ribonuclease).ThehumanhomologueofmouseCADwasmorerecentlyidentifiedandtermedCPAN,DFF40,andhumanCAD.DFF45/ICAD
istheinhibitoryproteinofDFF40/CADwithwhichitformscomplexes.Uponcleavagebyactivatedcaspase,DFF40/CADisreleasedandactivatedandeventuallycausesthedegradationofDNAinthenuclei.ActivationofDFF40/CAD,whichcausesDNAdegradation,isthehallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa203-218ofhumanDFF40(accessionno.NP_004393).HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratDFF40/CAD(40kDa).
APPLICATIONSImmunoblotting:useat1-2ug/ml.
WesternblotanalysisofDFF40inJurkatcelllysatewithDFF40antibodyat(A)0.5,(B)1and(C)2g/ml.
Positivecontrol:WholecelllysateofJurkatcells.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofDFF40inK562cellswithDFF40antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
