Anti-DFF40/CAD(NT)Antibody
CatalogNo.:2153
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.ThesedeathsignalsfinallycausethedegradationofchromosomalDNAbyactivatedDNase.AmouseDnasethatcausesDNAfragmentationwasidentifiedrecentlyanddesignatedCAD(forcaspaseactivateddeoxy-ribonuclease).ThehumanhomologueofmouseCADwasmorerecentlyidentifiedandtermedCPAN,DFF40,andhumanCAD.DFF45/ICAD
istheinhibitoryproteinofDFF40/CADwithwhichitformscomplexes.Uponcleavagebyactivatedcaspase,DFF40/CADisreleasedandactivatedandeventuallycausesthedegradationofDNAinthenuclei.ActivationofDFF40/CAD,whichcausesDNAdegradation,isthehallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa3-18ofhumanDFF40(accessionno.NP_004393).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanDFF40(40kDa).
APPLICATIONSImmunoblotting:useat2ug/ml.
WesternblotanalysisofDFF40inJurkat(J)andK562(K)wholecelllysatewithDFF40antibodyat2ug/ml.
Positivecontrol:WholecelllysateofK562orJurkatcells.
Immunocytochemistry:useat5ug/ml.
ImmunocytochemicalstainingofDFF40inJurkatcellswithDFF40antibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
