Anti-DNaseII(CT)Antibody
CatalogNo.:2059
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
DNaseIIcausesbothchromatincondensationandDNAfragmentation.Thegenesencodinghuman,porcine,andmurineDNaseIIhavebeencloned.TheDNaseIIgeneencodesa40kDaproenzyme.Thematureenzymeconsistsoftwonon-identicalsubunits,the32kDa()and12kDa()chains,generatedbyproteolyticprocessing.OverexpressionofDNaseIIinduceschromatincondensation.DNaseIIisubiquitouslyexpressedinhumantissues.
Antigen:Peptidecorrespondingtoaa347-360ofhumanDNaseIIprecursor(accessionno.AF047016).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizestheproenzymeofhumanDNaseII(40kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofDNaseIIinhumanspleentissuelysateintheabsence(lane1)orpresence(lane
2)ofblockingpeptidewithDNaseIIantibodyat1μg/ml.
Positivecontrol:Tissuelysateofhumanspleen.
Immunohistochemistry:useat5ugml.
ImmunohistochemicalstainingofDNaseIIinhumanspleenwithDNaseIIantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
