Anti-DR3(ED)Antibody
CatalogNo.:2401
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ApoptosisisinducedbycertaincytokinesincludingTNFandFasligandoftheTNFfamilythroughtheirdeathdomain-containingreceptors,TNFR1andFas.AnothercelldeathreceptorwasrecentlyidentifiedanddesignatedDR3,
Wsl-1,Apo-3,TRAMP,andLARD.DR3is
widelyexpressedintissuesenrichedinlymphocytesincludingperipheralblood,thymus,andspleen.LikeTNFR1,DR3inducesapoptosisandNF-Bactivation.
Antigen:PeptidecorrespondingtotheextracellulardomainofhumanDR3precursor(accessionno.AAQ88676).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhumanandmouseDR3(59kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofDR3inJurkatcelllysatewithDR3antibodyat1g/ml.
Positivecontrol:WholecelllysatefromJurkatcells.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofDR3inJurkatcellswithDR3antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
