Anti-DR4(CT)Antibody
CatalogNo.:2404
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ApoptosisisinducedbycertaincytokinesincludingTNFandFasligandoftheTNFfamilythroughtheirdeathdomain-containingreceptors,TNFR1andFas.AnothercelldeathreceptorwasrecentlyidentifiedanddesignatedDR4.TheligandforDR4hasbeendesignatedTRAILandisanewmemberoftheTNFfamily.Therefore,DR4isalsocalledTRAILreceptor-1.DR4isexpressedinmosthumantissuesincludingspleen,peripheralblood,smallintestine,andthymus.LikeTNFR1,FasandDR3,DR4mediatesTRAIL-inducedapoptosisandNF-Bactivationinmanytissuesandcells.
Antigen:Peptidecorrespondingtoaa427-445attheC-terminusofhumanDR4(accessionno.ACC51226).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhumanDR4(57kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofDR4inHeLacelllysatewithDR4antibodyat1μg/ml.
Positivecontrol:WholecelllysatefromHeLacells.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofDR4inJurkatcellswithDR4antibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
