CatalogNo.:1167

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

ApoptosisisinducedbycertaincytokinesincludingTNFandFasligandoftheTNFfamilythroughtheirdeathdomain-containingreceptors,TNFR1andFas.AnothercelldeathreceptorwasrecentlyidentifiedanddesignatedDR4(fordeathreceptor4).TheligandforDR4hasbeendesignatedTRAILandisanewmemberoftheTNFfamily.Therefore,DR4isalsocalledTRAILreceptor-1.DR4isexpressedinmosthumantissuesincludingspleen,peripheralblood,smallintestine,andthymus.LikeTNFR1,FasandDR3,DR4mediatesTRAIL-inducedapoptosisandNF-Bactivationinmanytissuesandcells.

Antigen:Peptidecorrespondingtoaa1-20ofhumanDR4matureprotein(accessionno.

ACC51226).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizeshumanDR4(57kDa).

Immunoblotting:useat2ug/ml.

WesternblotanalysisofDR4inHeLa(H),K562(K),andJurkat(J)wholecelllysatewithDR4antibody

at2ug/ml.

Positivecontrol:WholecelllysatefromHeLa,K562,orJurkatcells.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.