Anti-DR6(NT)Antibody
CatalogNo.:2157
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ApoptosisisinducedbycertaincytokinesincludingTNFandFasligandoftheTNFfamilythroughtheirdeathdomain-containingreceptors,TNF-R1andFas.SeveralnoveldeathreceptorsincludingDR3,DR4,andDR5wererecentlyidentified.Anewdeathdomain-containingreceptorintheTNFreceptorfamilywasrecentlydescribedanddesignatedDR6.LikeTNF-R1,DR6interactswithdeathdomain-containingadaptermoleculeTRADD.OverexpressionofDR6inducesapoptosisandactivatesNF-BandJNK.DR6iswidelyexpressedinhumantissuesandcelllines.TheligandforDR6isunderinvestigation.
Antigen:Peptidecorrespondingtoaa42-56ofhumanDR6precursor(accessionno.
AF068868).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanDR6(68kDa).
APPLICATIONSImmunoblotting:useat2ug/ml.
WesternblotanalysisofDR6inK562(1,3)andRaji(2,4)wholecelllysateintheabsence(1,2)orpresence(3,4)ofblockingpeptide
withanti-DR6(NT)at2ug/ml.
Positivecontrol:WholecelllysatefromK562orRajicells.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
