Anti-EndoGAntibody
CatalogNo.:11004
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:Peptidecorrespondingtoaa55-70ofhumanEndoG(accessionno.
NP_004426).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
Thisantibodyrecognizeshuman,mouse,andratEndoG(EndonucleaseG)anenzymethatisactivatedbyapoptoticstimuliandisabletoinducenucleosomalfragmentationofDNAindependentlyofcaspaseandDFF/CAD.EndoGisamitochondria-specificnucleasethattranslocatestothenucleusandcleaveschromatinDNAduringapoptosis.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1-2ug/ml.Abandof35kDaisdetected.
WesternblotanalysisofEndoGinmouse(M)3T3andhuman(H)HepG2celllysateswithEndoGantibodyat2μg/ml.
Positivecontrol:HepG2celllysate.
Immunohistochemistry:useat10-15ug/ml
ImmunohistochemicalstainingofEndoGinhumanpancreaswithEndoGantibodyat15μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
