Anti-F1AαAntibody
CatalogNo.:2279
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Fasandtumornecrosisfactorreceptor1(TNFR1)aretwomembersofthefamilyofdeathreceptors.AnovelproteinthatassociateswiththeintracellulardomainsofFasandTNFR1hasbeendesignated(orFEM1).F1AiscleavedbyCED-3andcaspase,anditassociateswithCED-4anditsmammalianhomologueApaf-1.
OverexpressionofF1Ainducesapoptosis.F1Aisexpressedinavarietyofhumanandmousetissues.
Antigen:Peptidecorrespondingtoaa609-622ofhumanF1A(accessionno.
AAF05314).Thissequenceisidenticaltothatofmouse.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
Thisantibodyrecognizeshuman,mouse,andratF1A(70kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofF1Aαinmouse(A)andrat(B)livertissuelysateswithF1Aαantibodyat1μg/ml.
Positivecontrol:Mouselivertissuelysate.
Immunohistochemistry:useat5ug/ml.
ImmunohistochemicalstainingofF1AαinmouselivertissuewithF1Aαantibodyat5ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
