Anti-FLIP(CT)Antibody
ORDERINGINFORMATION
CatalogNo.:2422
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Caspase-8(FLICE)and-10(FLICE2)aretwopivotalmembersoftheICE/CED-3proteasefamily.FLICE-inhibitoryproteinshavebeenidentifiedinvirusesandhumancellsandaredesignatedv-FLIPsandFLIP,respectively.HumanFLIPwasclonedbyseveralindependentlaboratoriesanddesignatedCasper,I-FLICE,FLAME-1,CASH,andCLARP.FLIPcontainstwodeatheffectordomainsandacaspase-likedomain.FLIPinteractswithadapterproteinFADDandcaspase-8and-10andpotentlyinhibitsapoptosisinducedbydeath
receptorsCD95,DR3,TRAIL-R,andTNFR1.Foursplicevariantsofc-FLIPshavebeenidentifiedandtermedFLIPα,β,γ,andδ,respectively.
Antigen:Peptidecorrespondingtoaa447-464attheC-terminusofhumanFLIPα/FLIPL(accessionno.AAC51622)
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhuman,mouse,andratFLIPα(55kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofFLIPinHeLa(H),Jurkat(J),andK562(K)wholecelllysatewithFLIPantibodyat1ug/ml.
Positivecontrol:WholecelllysatefromHeLacells.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofFLIPαinJurkatcellswithFLIPαantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
